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The Working Process of Chromatography
In all chromatography, people come across a stationary phase and a mobile phase. The mobile phase does move through the stationary phase collecting up the substances that need to be examined. The mobile phase while traveling through the stationary phase takes the substances with it.
On the other hand, the stationary phase happens to be the phase that does not move. At various points of the stationary phase, various substances of the compound get absorbed and they stop moving in the mobile phase. In this way, the outcomes of chromatography are acquired from the point where various constituents of the compound do not move and get separated from other components. BookMyEssay writers are ready to help you immediately when you search write my assignment for me on Chromatography Techniques.
In thin-layer and paper chromatography, the mobile phase becomes the solvent and in paper chromatography, the stationary phase is considered the thin-layer cell. These kinds of chromatography make use of capillary for moving the solvent via the stationary phase.
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Different Types of Chromatography
Chiefly, there are four significant types of chromatography and they are:
- Liquid Chromatography – It is used for testing water samples for looking for pollution in rivers and lakes. Liquid chromatography is also used for analyzing organic compounds and ions in solutions. This kind of chromatography makes use of liquids that might incorporate insoluble and hydrophilic molecules.
- Gas Chromatography – It is used in airports for detecting bombs and is used in forensics in various ways. This is useful for analyzing fibers on the body of a person and also analyzing blood which is found at the scene of a crime. Here, helium is useful for moving gaseous mixture via a column of absorbent component.
- Paper Chromatography – It is one of the highly common kinds of chromatography. This makes use of a strip of paper in the form of a stationary phase.
- Thin-layer Chromatography – This kind of chromatography makes use of absorbent components on plastic plates or flat glass. It is a rapid and simple process for checking the purity of the organic compounds.