The roles of the liver and pancreas to regulate the cholesterol esters levels in the blood have not been described. It is, therefore, necessary to investigate the characteristics and occurence of its activity in the liver along with the method that is developed in laboratories. If you avail of our online Cholesterol Esterase Assay assignment assistance, you can secure top grades.
Characteristics of Cholesterol Esterase
The characteristics of Cholesterol Esterase is discussed in our Cholesterol Esterase Assay homework writing service. Its features are different in structure, molecular size, reaction kinetics, and substrate specificity from different sources. Its relative molecular mass is between 12 kDa – 60kDa.
It has, however, a much larger molecular weight of more than 60kDa that is composed of over 500 amino acid residues. The molecular weight after detection through gel filtration can be a little high due to cholesterol acetase ploymersae formation. As per the amino acid sequence and the structural characteristics of the enzyme, it may be divided into two groups.
CHE from greater yeast, eukaryotes, bacteria source comprises a family that has the same catalytic center and has a GXSXG sequence along with a catalytic triplet such as hidtidine/serine/glutamic and aspartic acid. CHE hydrolysis catalytic mechanism is quite similar to chymotrypsin serine protease.
CHE from the actinomycetes belongs to another family and this family does not have any typical GXSXG sequence of an alcohol esterase, however, it has three amino acid residues: one aspartic acid and two serine residue. Our highly qualified and highly experienced experts can provide you with the highest quality assignment writing help on Cholesterol Esterase Assay.
Researchers have studied in the recent years that substrate specificity as well as reaction kinetics of CHE from microbial sources. Some differences are there among the various sources of CHE such as temperature, pH, substrate, stability, activity assay, and specificity. The activity of the enzyme is decided to depend on its absorbance value.
Cholesterol esterase sources are mentioned in our Cholesterol esterase Assay assignment help online. It is found in several biological tissues such as the pancreas, liver, gonad, adrenal gland, brain, breast, and corpus luteum. Its products come mostly from animal organs. In the 1960s, many researchers have focused their attention on cholesterol production by microorganisms.
The microorganisms are pseudomonas mendocina, pseudomonas fluorescent, strptomyces lavendulac, Alcaligenes sp, oxysporum, and others. In the year 1975, CHE was purified from pseudomonas cultures. It is known that various CHE sources have various functions and CHE in the liver, pancreatic, and light base were used and studied widely.