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Lipid peroxidation is considered the oxidative degradation of lipids and it tends to be a chain reaction that the inclusion of an oxygen radical or hydrogen abstraction initiated. This has resulted in the oxidative impairment of PUFA (polyunsaturated fatty acids).

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What is Meant by Lipid Peroxidation?

Lipid peroxidation is acknowledged as the method in which free radicals do steal electrons present in the cell membranes’ lipids. This results in cell damage. This method is preceded by a radical chain reaction process.

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Mostly, it affects PUFAs as they comprise several double bonds and in between lays methylene bridges. They own reactive hydrogen atoms. The reaction comprises three important steps; initiation, termination, and propagation.

The chemical components of this oxidation are called lipid oxidation productions or lipid peroxides. Students always come to us for getting Lipid Peroxidation Assay assignment help because our paper writers never make mistakes in sentence construction.

Knowing Lipid Peroxidation

Emotional and physical stress, carcinogenesis or inflammation, and metabolic alterations are some conditions that are capable of triggering oxidative stress. This is known as the balance shift of some redox reactions towards oxidation. This results in the augmentation of ROS (reactive oxygen species). ROS are formed continuously in little quantities at the time of the usual metabolism of the cell. Nonetheless, the overproduction of reactive oxygen species becomes cytotoxic and it injures macromolecules, like sugars, proteins, DNA, and lipids.

PUFAs that happen to be esterified in storage or membrane lipids remain subject to ROS-induced peroxidation, thus, results in the damaging of biomembranes. The final components of LPO (lipid peroxidation) tend to be reactive aldehydes which are comparatively stable and they might diffuse from the actual place of oxidative injury. Additionally, they might work as free radicals or second messengers. The difference between pathological and physiological oxidative stress is habitually the incidence of LPO besides its ultimate toxic components.

A couple of classes of processes for the extent of LPO are mentioned. The 1st comprises assays for detecting LPO at the level of organismal whereas the 2nd comprises cellular and molecular assays that disclose the mechanistic impacts of LPO on morphology, viability, and functions of the cells. We have got secure and safe payment methods and so, students do not think twice but take Lipid Peroxidation Assay assignment help online from us.

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How Can You Measure Lipid Peroxidation?

You can determine lipid peroxidation qualitatively or quantitatively by various processes. You can measure it by the loss of fatty acids, amounts of some secondary components, like hydrocarbon gases and carbonyls, amounts of chief peroxidation products, and lessening of antioxidant activities.

Some well-known processes are mentioned below:

  • GLC (gas liquid chromatography)
  • HPLC (high-performance liquid chromatography)

Lipid hydroperoxides which is the chief component of peroxidation can be measured directly by HPLC with the help of chemiluminescence detectors. Glutathione peroxidase and iodine liberation processes are habitually utilized to measure lipid peroxides.

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